All of my embedded figshare posts will be put in a "figshare" category under "Personal" just like this one, so if for some reason you want to find a figshare post in the future, it should be easy to do.
Recently in Personal Category
All of my embedded figshare posts will be put in a "figshare" category under "Personal" just like this one, so if for some reason you want to find a figshare post in the future, it should be easy to do.
But that's not the incident I refer to. No, the incident I refer to happened before we even got to where we could start our sampling.
We rented a 4x4 through Drive South Africa. We picked up a sturdy Nissan Double Cab 4x4 from KEA Camper Rentals at Cape Town International at 8:00am on a Friday and left on the N2 for El Yolo One. We stopped at the Peregrine Farm Stall in Grabouw to pick up something for lunch along the way. We continued on the N2 to just past Swellendam, where we turned off on the R324. We stopped for lunch in Tradouw Pass a little after noon and continued on our way. We joined the R62 at Barrydale and headed toward Ladismith. Then it happened.
About 30km south of Ladismith we driving along at about 100kph when we heard a loud "bang" and the truck started to lurch left. My immediate thought was that we'd blown a tire. It didn't make sense, because we hadn't hit anything. But I hit the brakes and guided us to the shoulder.
That's when our wheel came bouncing by on the left, glanced off our left front fender, and knocked down a nearby highway sign, snapping the wooden supports as if they were toothpicks. We got out and went around to the front left side to see what had happened, since we were obviously leaning that way. The whole wheel was missing. We'd driven on the brake pad for the 100m or so that it took me to get us stopped.
Some motorcyclists who were passing by saw what had happened and stopped to ask if we were OK. I was still pretty dazed and trying to figure out what to do when one of them pointed out that we could use lug nuts from the spare on back to put a wheel back on. Well, it turns out that the lug nuts holding the spare on weren't the same size as those that held on the wheels. Although it probably would have been fine to put the original wheel back on, I decided to use the spare -- and steal lug nuts from the other wheel.
I couldn't get any off the right front wheel, so I took two (of six) from the right rear wheel and one from the left rear wheel. There were only three usable bolts on the right front wheel. One had been sheared off, and the threads on two more were damaged. Unfortunately, the three usable lugs were adjacent to one another, so the wheel wasn't evenly balanced in its connection to the brake pad.
So I turned on the emergency flashers, and drove slowly into Ladismith, about 30kph. Jane called ahead and found us a place to stay. KEA sent a replacement vehicle that night, and after that we had no problems at all. Jane and Nora seemed amazed at my skill in keeping the car under control, but honestly, all I did was to hit the brake and steer us to the left shoulder. I wasn't thinking about anything, just acting instinctively.
Protea canaliculata at Teeberg in the Swartberg Pass, South Africa
Photograph by Kent Holsinger
Click on the image for a high-resolution image in a new window.
My personal bag and, more importantly, the duffle with DNA samples arrived with me. The trip was a great success.
The focus of this trip was to collect samples in a hybrid zone between Protea punctata and Protea venusta at Blesberg and to collect additional samples of both species in areas of Swartberg Pass where they are not known to be hybridizing.
Nora will use the samples to unravel the dynamics of this hybrid zone. She left dataloggers in place at Blesberg to record temperature and humidity for the next year. She'll use the results from analysis of RAD SNPs (more on that in a moment) to develop a hybrid index, look at performance of individuals along the steep environmental gradient, and relate individual performance to traits. In addition, she collected seed for half-sib families which she'll use for paternity analysis to determine whether there are asymmetries in gene exchange and to identify the causes of any asymmetry she detects.
The hybrid zone is also of great interest for the Dimensions of Biodiversity project. Justin will use RAD sequencing to identify a large number of polymorphic SNPs. We'll use those SNPs as markers in a genome-wide association study to dissect the genetic basis of traits that distinguish these two very different species.
Protea venusta at Blesberg, ca. 20km east of Klaarstoom, South Africa
Photograph by Kent Holsinger
Click on the image for a high-resolution image in a new window.
On the first day of work at Blesberg, I know that I slowed things down, because I'm not as sure on my feet as Nora and Jane. On the second day, I started down again and was making progress slowly. But as I was clambering down a particularly steep pitch about 150m from the top my legs started to give out. It wasn't just that I was moving slowly. I was afraid that if I descended any further, I wasn't going to make it back to the top. I asked Nora and Jane to go on without me. I climbed back to the top by myself -- slowly -- and waited for them to return.
For the next two days I didn't even try to make the descent and help. I collected some samples of Protea montana and looked for more than the single individual of Protea pruinosa -- without success. But Nora and Jane made the trip quicker every day, even though the conditions got worse.
Nora (on the left) and Jane (on the right) heading down from the top of Blesberg on one of those days where I wimped out and stayed on top.
Photograph by Kent Holsinger
Click on the image for a high-resolution image in a new window.
The next day, we were planning a longer, 14km hike to Oliewensberg (7km each way), but we passed a policeman on our way up to the pass, and when we arrived at Teeberg, we decided to turn around. The road was covered with ice and snow. I'm sure our 4x4 would have made it, but we were 200m or so below the summit, and the hiking conditions would have been even worse.
We left Prince Albert the next morning making contingency plans, since we didn't think the snow would have melted and we didn't expect to be able to collect samples. To our surprise, the drive to the summit was pretty easy, so we started up the path. At first, the going wasn't bad, occasional patches of snow, but the trail was mostly bare, although it was a bit wet.
Once again it became clear that I was going to slow Nora and Jane down, but since we had plenty of time, and I was only slowing them down a little, we kept going. At about kilometer 3, though, things changed. The going got very steep, which would have been fine had the trail been clear. Unfortunately, it was covered in 10-15cm of heavy, wet snow. I was climbing through it -- very, very slowly. Nora and Jane waited for me at the top of an intermediate summit, and just before I got there, I looked at my watch. We'd come about 3.5km in an hour, and we had another 3.5km to go -- under worse conditions. This time I could have made it, but I was moving so slowly because of my unsteadiness, it would have taken us another couple of hours to get to where we needed to sample. And once I arrived, I wouldn't have been able to help. I told them to go on without me, and I went back to the summit and waited in the car.
In the end, Nora and Jane got a lot of really good samples. We even picked up several taxa for the trait-environment analysis in Dimensions that we hadn't sampled before. So it was a very successful trip. At the same time, I began to wonder about my future in South Africa during the flight home.
Nora needs someone dependable, i.e. someone other than me, to help her when she goes back next year. We've collected samples and trait data from all of the taxa it's reasonable to imagine we can get for our trait-environment analyses. Jane doesn't need me as a field assistant for her polymorphism work. I can't think of a good reason that anyone should spend good money to get me to South Africa next year.
I don't like thinking this, but I have to wonder whether 2013 may have been my swan song.
Here's what I had to say:
Other stories from the Daily Digest for Monday, April 22, 2013 >>
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Next 6 hoursSo far there's no evidence of the winds, but the current temperature is 26°F - a beautiful December day.
Variable clouds with strong and gusty winds. Temperatures steady or falling to near 22F. Winds WNW at 25 to 30 mph.

English: Hardy-Weinberg principle for two alleles (Photo credit: Wikipedia)



